Some of the most popular applications at our facility include distance, area, volume, voxel intensity, and co-localization statistics.
In addition to the valuable images generated with Imaris®, a variety of statistical data can also be acquired. The Keyframe Animator Module of Imaris® generates incredible movies. The Snapshot tool allows one to image various views of samples, including x, y, and z views as well as gallery and 3D rotational views. The combination of all these tools, which take a matter of minutes to use, will result in a dataset that can provide amazing pictures. Colors can be changed and made transparent with ease for optimal visualization. Thresholding and Background Subtraction can also be used to refine the dataset. Imaris® offers smoothing capabilities with Gaussian and Median filters, which eliminate excessive pixilation. Upon loading a dataset in Imaris®, it is easy to process the images for a spectacular three-dimensional rendering. This provides for a refined dataset and ultimately a more precise evaluation of protein expression patterns. Imaris® not only supports the file formats of these microscopes but it enables the user to quickly resample or crop large datasets directly from the original file. Four-color immunofluorescence on cell culture/tissue samples is in high demand, and having Zeiss LSM 510 and Nikon PCM-2000 Laser Confocal Scanning Microscopes provides for very complex datasets. The possibilities are endless with regards to the capabilities of Imaris®. Some of the formats include Tiff series, BMP series, Leica, Olympus, and Zeiss datasets. Bitplane AG has created Imaris®, a program that supports images acquired from almost all microscope manufacturers.
This can result in the daunting task of finding something that will support the file format as well as provide the proper analysis tools. There is a variety of microscopes in the market and almost all have acquisition software that saves images in a different format. With the advent of confocal microscopes and 3D/4D image acquisition capabilities, image processing and analysis can be overwhelming.
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